| Congresso Brasileiro de Microbiologia 2023 | Resumo: 378-1 | ||||
Resumo:Pathogenic and multidrug-resistant (MDR) Enterobacterales strains represent a serious public health problem due to the limitation of antimicrobial options against severe infections caused by them. Enterobacterales strains have been circulating at the human-animal-environment interface. In this context, the environment can spread MDR Enterobacterales strains and their antimicrobial resistance genes (ARGs). Therefore, this study aimed to characterize the antimicrobial resistance of Enterobacterales strains obtained from different aquatic ecosystems. Eighty-six water samples were collected from 58 cities in the state of São Paulo, Brazil, and were filtered by the filtering membrane technique. Subsequently, the membranes were placed on MacConkey agar supplemented with 4 mg/L of ceftriaxone or colistin. Accordingly, seventeen Escherichia coli, ten Serratia marcescens, and one Enterobacter cloacae complex were identified using the sequencing of 16S rRNA. The disk diffusion and broth microdilution methods were performed to determine the antimicrobial susceptibility. In addition, the ARGs and plasmid replicons were investigated by conventional polymerase chain reactions. In general, all strains exhibited resistance to at least one agent of the aminoglycoside class, while all E. coli strains were classified as MDR since exhibited resistance to β-lactams, fluoroquinolones, sulfonamides, tetracyclines, polymyxins, and/or phenicols. Furthermore, only three S. marcescens strains were MDR, presenting resistance to streptomycin, gentamicin, tetracycline, and trimethoprim-sulfamethoxazole agents. Several ARGs were found, highlighting the presence of extended-spectrum β-lactamase (ESBL)-encoding genes (e.g., blaCTX-M and blablaGES) in E. coli. Among the other species, various aminoglycoside resistance genes were found, spotlighting the aac(6')-Ib gene in all strains. Furthermore, different plasmid families were detected, including IncR, IncFIA, and IncP. Therefore, this study calls attention to the occurrence of Enterobacterales MDR and ESBL-producing E. coli strains in water samples, which are recognized as a critical priority by the World Health Organization. Palavras-chave: Enterobacter cloacae complex, Escherichia coli, Extended-spectrum β-lactamase, Serratia marcescens, Water Agência de fomento:FAPESP (2021/01655-7), CAPES (88887.519091/2020-00, 8887.824722/2023-00, and Finance Code 001), and CNPq (304905/2022-4, 308914/2019-8, 130086/2021-5, 141016/2021-3, and 150712/2022-7). | |||||